Introduction

Citrus fruits (including tangerines) are one of the most widely cultivated fruits. Citrus fruits contain a whole series of useful bioactive compounds such as essential oils, polyphenols, carotenoids and vitamins. In the tangerine juice industry, the residual part is about 60% of the citrus mass, which consists of peels, seeds, mesocarp and pulp. However, landfilling these waste products without further treatment can cause serious environmental problems. At the same time, this non-edible secondary biomass contains a large amount of bioactive compounds, which makes it an ideal sustainable and renewable raw material for the production of high-value-added chemicals required by the food, pharmaceutical and cosmetic industries.

The aim of the project is to develop and optimize mandarin juice pomace recovery technologies to reduce the environmental footprint of the Georgian mandarin juice industry using circular economy principles. The research focuses on sustainable and effective extraction methods such as steam distillation (for limonene extraction) and ultrasound-assisted extraction (for hesperidin extraction). The goal of developing technologies for the maximum recovery of mandarin juice press residues is the gradual scaling of technology transfer from the laboratory to industry and the application of the technology in an industrial process.

Membership

Dr. Maria Kuhtinskaya Group leader
Dr. Merike Vaher  Group leader
Dr. Maria Kulp
Dr. Tiit Lukk
Marlene Leemet Master's student
Annabel Taniel Undergraduate student

Alumni

Dr. Piret Saar-Reismaa

Technical ability

Extraction:

  • Ultrasonic assisted extraction device, VWR
  • Steam distillation apparatus (upon acquisition)

Analysis:

  • 7100 Capillary Electrophoresis with Diode-Matrix UV Detector (CE-UV-DAD), Agilent Technologies
  • 1200 Infitity Liquid Chromatograph with UV-DAD and Mass Spectrometry Detector (MSD Trap XCT), Agilent Technologies
  • 1260 Infinity II liquid chromatograph with fluorescence detector, Agilent Technologies
  • 7890A Gas chromatograph with flame and mass spectrometric detector (5975C MSD quadrupole mass spectrometer), Agilent Technologies
  • Bruker Avance III 400 MHz FT-NMR spectrometer
  • MB120 moisture meter, Ohaus
  • And other smaller equipment

Isolation and cleaning:

  • Flash chromatograph, Biotage, Isolera Prime (Columns: Spär C18 D – Duo 100 Å 30 μm 12 g, 2/cs; Spär C18 D – Duo 100 Å 30 μm 30 g, 2/cs; Spär C18 D – Duo 100 Å 30 μm 60 g, 2/cs

Current projects

KIK21023 – Development of technologies for beneficiation of mandarin juice press residues with the aim of reducing the environmental footprint of Georgian fruit juice industries using circular economy principles (Climate. 3.01.20-0082). (ETIS e-profile, KIK project introduction)

The project is financed by the Ministry of the Environment from the funds of international climate cooperation.