Introduction

Our research is focused on global biological sustainability challenges, including the sustainable production of food and feed, as well as biochemicals and materials. In our work, we develop new bio-based processes where we use cell factories to convert various organic wastes, such as food and wood industry waste, into value-added products.

Based on the multidisciplinary skills of our research team, we have created a Design-Build-Test-Learn cycle for cell factory design and bioprocess optimization. We use metabolic modeling to create new cell factories; we develop new synthetic biology tools to more efficiently engineer cell factories; and use our lab-scale bioreactor platform to characterize and optimize the process. In addition, we use 3D printing to develop “living materials” that improve biotech-based manufacturing processes.

By combining these approaches, we aim to translate basic science results into industrial biotechnology applications by building more efficient producer cells. Together with our international and local partners, we develop all value chains of the circular economy to ensure the sustainable production of value-added products with minimal waste flow.

More information about the working group can be found at: bioeng.taltech.ee

Membership

Prof. Petri-Jaan Lahtvee Group leader
Nemailla Bonturi Senior researcher
Rahul Kumar Senior researcher
Sadia Khalid Engineer
Srdjan Gavrilovic Scientist
Paola Monteiro Doctoral student
Alina Rekena Doctoral student
Inna Lipova Doctoral student
Henrique Sepulveda Doctoral student
Juliano Sabedotti De Biaggi Scientist

Technical ability

Cells in silico design

  • Generating enzyme-constrained genome-wide metabolic models

Construction of cells

  • CRISPR-Cas9 and Golden Gate Assembly methodologies
  • Gel electrophoresis
  • PCR instruments

Cultivation platform

  • 3x 1L bioreactors (Applikon), with on-line gas analysis
  • 17x 40mL bioreactors (D2Biotech and Chi-bio)

Analytics

  • Shimadzu Prominence LC-2030C Plus liquid chromatograph for the analysis of sugars, carotenoids, organic and amino acids.

Current projects

DIGIBIO Center for Digitization of Biology - Next Generation Sustainable Products (European Commission, ETIS)

YAF  Yeast-based solutions for sustainable aviation fuel production (European Commission, ETIS)

RE.4.09.22-0041 Biotechnological recovery of wood and food waste into feed and food supplements (SA Keskkonnainvestmentite Keskus, KIK)

PRG1101  Novel 3D printable cell factories for the production of oleochemicals (Etag, ETIS)

VNF21012 Sustainable production of aquaculture feed from residual products of the wood industry (NordForsk, ETIS)

VFP21014 High efficiency bio-based functional coating materials for wood and decorative applications (Bio-based Industries Joint Undertaking, ETIS)